Objectives

A student passing the course
- understands the types of variation in proteins caused by the biological and non-biological reasons.
- understands the need for purified proteins, and the level of purity and the amount of protein required in different applications.
- knows the key principles the protein purification processes are based on.
- knows the most important techniques used to purify proteins and understands their principle of function.
- knows how to concentrate, handle and store proteins.
- can design a purification process for a specific protein.
- knows the functional principles of the most important methods used to analyse protein size, purity, interaction kinetics, isoelectric point, primary structure, stability, aggregation tendency.
- can select an appropriate analytical technique to obtain required information about a protein of interest.
- understands the function of most essential expression systems used for the recombinant protein production.
- understands the composition of the expression vectors used for the recombinant protein production.

Working life skills:
- presentation skills
- teamwork
  • Teacher: Anssi Malinen